Repo-Man coordinates chromosomal reorganization with nuclear envelope reassembly during mitotic exit.

Journal article

Repo-Man targets protein phosphatase 1 γ (PP1γ) to chromatin at anaphase onset and regulates chromosome structure during mitotic exit. Here, we show that a Repo-Man:PP1 complex forms in anaphase following dephosphorylation of Repo-Man. Upon activation, the complex localizes to chromosomes and causes the dephosphorylation of histone H3 (Thr3, Ser10, and Ser28). In anaphase, Repo-Man has both catalytic and structural functions that are mediated by two separate domains. A C-terminal domain local...

Expand abstract

Authors: Vagnarelli, P; Ribeiro, S; Sennels, L; Sanchez-Pulido, L; de Lima Alves, F

• Publication status: Published
• Journal: Developmental cell
• Volume: 21
• Issue: 2
• Pages: 328-342
• Publication date: 2011-08-01
• DOI: 10.1016/j.devcel.2011.06.020
• EISSN: 1878-1551
• ISSN: 1534-5807
• Language: English
• Keywords: Green Fluorescent Proteins; Nuclear Proteins; Cell Line, Transformed; Mitosis; Carrier Proteins; Tandem Mass Spectrometry; Anaphase; Nuclear Envelope; Receptors, Neuropeptide Y; Protein Structure, Tertiary; Cyclin B; Phosphorylation; Models, Biological; Transfection; Chromosomes; Humans; RNA Interference; Histones; Protein Binding; beta Karyopherins; Cell Cycle Proteins; Gene Expression Regulation