Spliceosome-mediated decay (SMD) regulates expression of nonintronic genes in budding yeast.

Journal article

We uncovered a novel role for the spliceosome in regulating mRNA expression levels that involves splicing coupled to RNA decay, which we refer to as spliceosome-mediated decay (SMD). Our transcriptome-wide studies identified numerous transcripts that are not known to have introns but are spliced by the spliceosome at canonical splice sites in Saccharomyces cerevisiae. Products of SMD are primarily degraded by the nuclear RNA surveillance machinery. We demonstrate that SMD can significantly do...

Expand abstract

Authors: Volanakis, A; Passoni, M; Hector, R; Shah, S; Kilchert, C

• Publication status: Published
• Journal: Genes and development
• Volume: 27
• Issue: 18
• Pages: 2025-2038
• Publication date: 2013-09-01
• DOI: 10.1101/gad.221960.113
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: RNA Splicing; Mutation; Transcription Factors; Saccharomyces cerevisiae Proteins; Spliceosomes; Phenotype; Saccharomyces cerevisiae; Transcriptome; RNA Stability; RNA; Gene Expression Regulation, Fungal