Journal article icon

Journal article

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM

Abstract:

Monitoring of different signalling enzymes in a single assay using multiplex biosensing provides a multidimensional workspace to elucidate biological processes, signalling pathway crosstalk, and determine precise sequence of events at the single living cell level. In this study, we interrogate the complexity in cAMP/PKA-MAPK/ERK1&2 crosstalk by using multi-parameter biosensing experiments to correlate biochemical activities simultaneously in time and space. Using a single excitation wavel...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1038/srep41026

Authors


Demeautis, C More by this author
Sipieter, F More by this author
Chapuis, C More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, NDM, Human Genetics Wt Centre
Expand authors...
More from this funder
Funding agency for:
Padilla-Parra, S
More from this funder
Funding agency for:
Padilla-Parra, S
Publisher:
Nature Publishing Group Publisher's website
Journal:
Scientific Reports Journal website
Volume:
7
Publication date:
2017-01-20
Acceptance date:
2016-12-14
DOI:
EISSN:
2045-2322
ISSN:
2045-2322
Pubs id:
pubs:674998
URN:
uri:c2ed14dc-adce-4aca-989c-456720705be9
UUID:
uuid:c2ed14dc-adce-4aca-989c-456720705be9
Local pid:
pubs:674998

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP