Novel Plasmodium vivax dhfr alleles from the Indonesian Archipelago and Papua New Guinea: association with pyrimethamine resistance determined by a Saccharomyces cerevisiae expression system.

Journal article

In plasmodia, the dihydrofolate reductase (DHFR) enzyme is the target of the pyrimethamine component of sulfadoxine-pyrimethamine (S/P). Plasmodium vivax infections are not treated intentionally with antifolates. However, outside Africa, coinfections with Plasmodium falciparum and P. vivax are common, and P. vivax infections are often exposed to S/P. Cloning of the P. vivax dhfr gene has allowed molecular comparisons of dhfr alleles from different regions. Examination of the dhfr locus from a...

Expand abstract

Authors: Hastings, MD; Maguire, J; Bangs, M; Zimmerman, P; Reeder, J

• Publication status: Published
• Journal: Antimicrobial agents and chemotherapy
• Volume: 49
• Issue: 2
• Pages: 733-740
• Publication date: 2005-02-01
• DOI: 10.1128/aac.49.2.733-740.2005
• EISSN: 1098-6596
• ISSN: 0066-4804
• Language: English
• Keywords: Drug Resistance; Folic Acid Antagonists; Indonesia; Tetrahydrofolate Dehydrogenase; Alleles; Papua New Guinea; Pyrimethamine; Plasmodium vivax; Antimalarials; Saccharomyces cerevisiae; Microbial Sensitivity Tests; Codon; Animals; Cloning, Molecular