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In vivo and in situ replication labeling methods for super-resolution structured illumination microscopy of chromosome territories and chromatin domains

Abstract:

Recent advances in super-resolution microscopy enable the study of subchromosomal chromatin organization in single cells with unprecedented detail. Here we describe refined methods for pulse-chase replication labeling of individual chromosome territories (CTs) and replication domain units in mammalian cell nuclei, with specific focus on their application to three-dimensional structured illumination microscopy (3D-SIM). We provide detailed protocols for highly efficient electroporation-based d...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Department:
Oxford, MSD, Biochemistry
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Department:
Oxford, MSD, Biochemistry
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Department:
Oxford, MSD, Biochemistry
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Grant:
Strategic Award 091911
Publisher:
Springer New York Publisher's website
Journal:
Methods in Molecular Biology Journal website
Volume:
1431
Pages:
127-140
Publication date:
2016
Acceptance date:
2016-05-17
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Pubs id:
pubs:628591
URN:
uri:c206c601-7710-488f-9a8e-4dd6eeb4e9b0
UUID:
uuid:c206c601-7710-488f-9a8e-4dd6eeb4e9b0
Local pid:
pubs:628591
ISBN:
978-1-4939-3629-8

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