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Quantitative in vivo measurement of glutathione in Arabidopsis cells.

Abstract:

A new, non-destructive assay is described to quantify cytoplasmic glutathione (GSH) levels in vivo in single cells or populations of cells from Arabidopsis suspension cultures. Cytoplasmic GSH was labelled with monochlorobimane (MCB) in situ to give a fluorescent GSH-bimane (GSB) conjugate. At low (10-100 microM) concentrations of MCB, labelling was mediated by a glutathione S-transferase, which confers specificity for GSH. HPLC analysis of MCB-labelled low molecular-weight thiols showed that...

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Publication status:
Published

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Authors


Fricker, M More by this author
Journal:
The Plant journal : for cell and molecular biology
Volume:
27
Issue:
1
Pages:
67-78
Publication date:
2001-07-05
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
URN:
uuid:c1ee7858-6e44-4c4c-b496-2d5f4b402173
Source identifiers:
53068
Local pid:
pubs:53068

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