Interplay between lysine methylation and Cdk phosphorylation in growth control by the retinoblastoma protein.

Journal article

As a critical target for cyclin-dependent kinases (Cdks), the retinoblastoma tumour suppressor protein (pRb) controls early cell cycle progression. We report here a new type of regulation that influences Cdk recognition and phosphorylation of substrate proteins, mediated through the targeted methylation of a critical lysine residue in the Cdk substrate recognition site. In pRb, lysine (K) 810 represents the essential and conserved basic residue (SPXK) required for cyclin/Cdk recognition and p...

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Authors: Carr, S; Munro, S; Kessler, B; Oppermann, U; La Thangue, N

• Journal: EMBO J
• Volume: 30
• Issue: 2
• Pages: 317-327
• Place of publication: England
• Publication date: 2011-01-19
• DOI: 10.1038/emboj.2010.311
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Phosphorylation; Immunoblotting; Cell Cycle; Chromatin Immunoprecipitation; Humans; Protein Binding; Lysine; Retinoblastoma Protein; Luciferases; Flow Cytometry; Cyclin-Dependent Kinases; Methylation; Mass Spectrometry; Cell Line, Tumor; Models, Molecular; Immunoprecipitation