Nuclear magnetic resonance spectroscopy-based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions.

Journal article

Polyamines are ubiquitous aliphatic amines that have been implicated in myriad processes, but their precise biochemical roles are not fully understood. We have carried out metabolite profiling analyses of transgenic tomato (Solanum lycopersicum) fruit engineered to accumulate the higher polyamines spermidine (Spd) and spermine (Spm) to bring an insight into the metabolic processes that Spd/Spm regulate in plants. NMR spectroscopic analysis revealed distinct metabolite trends in the transgenic...

Expand abstract

Authors: Mattoo, A; Sobolev, A; Neelam, A; Goyal, R; Handa, A

• Journal: Plant physiology
• Volume: 142
• Issue: 4
• Pages: 1759-1770
• Publication date: 2006-12-01
• DOI: 10.1104/pp.106.084400
• EISSN: 1532-2548
• ISSN: 0032-0889
• Language: English
• Keywords: Fruit; Signal Transduction; Plants, Genetically Modified; Gene Expression Regulation, Plant; RNA, Messenger; Carbon; Carbohydrate Metabolism; Nitrogen; Lycopersicon esculentum; Nuclear Magnetic Resonance, Biomolecular; Choline; Amino Acids; Spermidine; Spermine; Isocitrate Dehydrogenase; Phosphoenolpyruvate Carboxylase