Detailed structural features of lipopolysaccharide glycoforms in nontypeable Haemophilus influenzae strain 2019.

Journal article

We have investigated the structure of the lipopolysaccharide (LPS) of nontypeable Haemophilus influenzae (NTHi) strain 2019, a prototype strain that is used for studies of NTHi biology and disease. Analysis of LPS from wild type and lex2B, lpt3 and pgm mutant strains using NMR techniques and ESI-MS on O-deacylated LPS and core oligosaccharide material (OS), as well as ESI-MS(n) on permethylated dephosphorylated OS, confirmed the previously established structure in which lactose is linked to t...

Expand abstract

Authors: Engskog, M; Deadman, M; Li, J; Hood, D; Schweda, E

• Journal: Carbohydrate research
• Volume: 346
• Issue: 10
• Pages: 1241-1249
• Publication date: 2011-07-01
• DOI: 10.1016/j.carres.2011.04.030
• EISSN: 1873-426X
• ISSN: 0008-6215
• Language: English
• Keywords: Spectrometry, Mass, Electrospray Ionization; Phosphorylation; Haemophilus Infections; Lipopolysaccharides; Hydrolysis; Carbohydrate Sequence; Haemophilus influenzae; Acetylation; Molecular Sequence Data; Glycosylation; Humans