Journal article icon

Journal article

Use of fluorescently labelled deoxyribonuclease I to spatially measure G-actin levels in migrating and non-migrating cells.

Abstract:

Lamellipodium protrusion is linked to actin filament disassembly in migrating fibroblasts [Cramer, 1999: Curr. Biol. 9:1095-1105]. To further study this relationship, we have identified a method to specifically and sensitively detect G-actin in distinct spatial locations in motile cells using deoxyribonuclease I (DNase I). Although DNase I can bind both G- and F-actin in vitro [Mannherz et al., 1980: Eur. J. Biochem. 95:377-385], when cells were fixed in formaldehyde and permeabilized in dete...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/cm.10013

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
Cell motility and the cytoskeleton
Volume:
51
Issue:
1
Pages:
27-38
Publication date:
2002-01-05
DOI:
EISSN:
1097-0169
ISSN:
0886-1544
URN:
uuid:c13b4dcb-1329-41e3-993c-0f185a5af77c
Source identifiers:
28384
Local pid:
pubs:28384

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP