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Two dimensional gel electrophoresis using narrow pH 3-5.6 immobilised pH gradient strips identifies potential novel disease biomarkers in plasma or serum

Abstract:

Two-dimensional gel electrophoresis (2-DE) is a protein separation technique often used to separate plasma or serum proteins in an attempt to identify novel biomarkers. This protocol describes how to run 2-DE gels using narrow pH 3-5.6 immobilised pH gradient strips to separate 2 mg of serum proteins. pH 3-6 ampholytes are used to enhance the solubility of proteins in this pH range before the serum proteins are separated in the first dimension by isoelectric point (isoelectric focusing) follo...

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Publication status:
Published
Peer review status:
Not peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1038/protex.2011.261

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Publisher:
Nature Publishing Group Publisher's website
Journal:
Protocol Exchange Journal website
Publication date:
2011-10-11
DOI:
EISSN:
2043-0116
URN:
uuid:c12f985f-8358-49f9-ab9a-3d2191c6f61d
Source identifiers:
207610
Local pid:
pubs:207610

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