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Physical and chemical considerations of damage induced in protein crystals by synchrotron radiation: a radiation chemical perspective.

Abstract:

Radiation-induced degradation of protein or DNA samples by synchrotron radiation is an inherent problem in X-ray crystallography, especially at the 'brighter' light sources. This short review gives a radiation chemical perspective on some of the physical and chemical processes that need to be considered in understanding potential pathways leading to the gradual degradation of the samples. Under the conditions used for X-ray crystallography at a temperature of <100 K in the presence of cryo...

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Publication status:
Published

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Publisher copy:
10.1107/s0909049502014553

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Oncology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author
Host title:
Journal of synchrotron radiation
Volume:
9
Issue:
Pt 6
Pages:
329-332
Publication date:
2002-11-01
Event location:
Denmark
DOI:
EISSN:
1600-5775
ISSN:
0909-0495
Keywords:
Pubs id:
pubs:100722
UUID:
uuid:c0dab9dd-dae4-4d12-bd4c-3a89f1d8abcc
Local pid:
pubs:100722
Source identifiers:
100722
Deposit date:
2012-12-19

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