Journal article icon

Journal article

Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching

Abstract:

We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these expe...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1007/s00339-007-4144-0

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Expand authors...
Journal:
APPLIED PHYSICS A-MATERIALS SCIENCE and PROCESSING
Volume:
88
Issue:
2
Pages:
223-226
Publication date:
2007-08-01
DOI:
EISSN:
1432-0630
ISSN:
0947-8396
Source identifiers:
222211
Language:
English
Pubs id:
pubs:222211
UUID:
uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2
Local pid:
pubs:222211
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP