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Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching

Abstract:

We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these expe...

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Publication status:
Published

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Publisher copy:
10.1007/s00339-007-4144-0

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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Role:
Author
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Journal:
APPLIED PHYSICS A-MATERIALS SCIENCE and PROCESSING
Volume:
88
Issue:
2
Pages:
223-226
Publication date:
2007-08-05
DOI:
EISSN:
1432-0630
ISSN:
0947-8396
URN:
uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2
Source identifiers:
222211
Local pid:
pubs:222211
Language:
English

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