- Abstract:
-
We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these expe...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1007/s00339-007-4144-0
-
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- Journal:
- APPLIED PHYSICS A-MATERIALS SCIENCE and PROCESSING
- Volume:
- 88
- Issue:
- 2
- Pages:
- 223-226
- Publication date:
- 2007-08-05
- DOI:
- EISSN:
-
1432-0630
- ISSN:
-
0947-8396
- URN:
-
uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2
- Source identifiers:
-
222211
- Local pid:
- pubs:222211
- Language:
- English
- Copyright date:
- 2007
Journal article
Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching
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