Journal article
Resolution of lambda/10 in fluorescence microscopy using fast single molecule photo-switching
- Abstract:
-
We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these expe...
Expand abstract
- Publication status:
- Published
Actions
Access Document
- Publisher copy:
- 10.1007/s00339-007-4144-0
Why is the content I wish to access not available via ORA?
Bibliographic data (the information relating to research outputs) and full-text items (e.g. articles, theses, reports, etc.) arrive in ORA from several different sources. Unfortunately we are not able to make available the full-text for every research output.
Please contact the ORA team (
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
) if you have queries regarding unavailable content OR if you are aware of a full-text copy we can make available.
Content may be unavailable for the following four reasons
-
Version unsuitable
-
We have not obtained a suitable full-text for a given research output. See this page for more information.
-
Recently completed
-
Sometimes content is held in ORA but is unavailable for a fixed period of time to comply with the policies and wishes of rights holders.
-
Permissions
-
All content made available in ORA should comply with relevant rights, such as copyright. See this page for more information.
-
Clearance
-
Some thesis volumes scanned as part of the digitisation scheme funded by Dr Leonard Polonsky are currently unavailable due to sensitive material or uncleared third-party copyright content. We are attempting to contact authors whose theses are affected.
Alternative access to the full-text
You may be able to access the full-text directly from the publisher's website using the 'Publisher Copy' link in the 'Links & Downloads' box from a research output's ORA record page. This method may require an institutional or individual subscription to the journal/resource.
Authors
Bibliographic Details
- Journal:
- APPLIED PHYSICS A-MATERIALS SCIENCE and PROCESSING
- Volume:
- 88
- Issue:
- 2
- Pages:
- 223-226
- Publication date:
- 2007-08-01
- DOI:
- EISSN:
-
1432-0630
- ISSN:
-
0947-8396
- Source identifiers:
-
222211
Item Description
- Language:
- English
- Pubs id:
-
pubs:222211
- UUID:
-
uuid:c063d22b-d179-4825-90d1-1b2f48bbb5d2
- Local pid:
- pubs:222211
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2007
If you are the owner of this record, you can report an update to it here: Report update to this record