Effects of troponin C isoform on the action of the cardiotonic agent EMD 57033.

Journal article

The effects of the cardiotonic potentiator EMD 57033 on different TnC (troponin C) isoforms were investigated. Endogenous skeletal TnC was extracted from glycerinated, permeabilized rabbit psoas fibres and replaced with either purified native rabbit psoas TnC (fast TnC) or human recombinant cTnC (cardiac TnC) (3 mg/ml in relaxing solution for 30 min). In both conditions, 10 microM EMD 57033 increased maximal calcium-activated force (Pmax) and gave a leftward shift in the pCa-tension curve. Wi...

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Authors: Lipscomb, S; Preston, L; Robinson, P; Redwood, C; Mulligan, I

• Publication status: Published
• Journal: Biochemical journal
• Volume: 388
• Issue: Pt 3
• Pages: 905-912
• Publication date: 2005-06-01
• DOI: 10.1042/bj20041841
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Protein Isoforms; Female; Protein Binding; Thiadiazines; Muscle, Skeletal; Phosphodiesterase Inhibitors; Troponin C; Quinolines; Cardiotonic Agents; Animals; Calcium; Rabbits