- Abstract:
-
A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formation and thus improve routine handling of the enzyme, facilitates crystallization by the hanging-drop vapour-diffusion technique. Crystals of (C334A)P450cam grow within 48 h and diffract to beyond 1.2 A a...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1107/s0907444997012195
-
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- Journal:
- Acta crystallographica. Section D, Biological crystallography
- Volume:
- 54
- Issue:
- Pt 3
- Pages:
- 470-472
- Publication date:
- 1998-05-05
- DOI:
- EISSN:
-
1399-0047
- ISSN:
-
0907-4449
- URN:
-
uuid:c01b4177-3c4d-4722-ab67-78bdc536d417
- Source identifiers:
-
36233
- Local pid:
- pubs:36233
- Language:
- English
- Keywords:
- Copyright date:
- 1998
Journal article
An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.
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