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An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.

Abstract:

A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formation and thus improve routine handling of the enzyme, facilitates crystallization by the hanging-drop vapour-diffusion technique. Crystals of (C334A)P450cam grow within 48 h and diffract to beyond 1.2 A a...

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Publication status:
Published

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Publisher copy:
10.1107/s0907444997012195

Authors


Nickerson, D More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Journal:
Acta crystallographica. Section D, Biological crystallography
Volume:
54
Issue:
Pt 3
Pages:
470-472
Publication date:
1998-05-05
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
URN:
uuid:c01b4177-3c4d-4722-ab67-78bdc536d417
Source identifiers:
36233
Local pid:
pubs:36233

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