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Resolving genetic functions within microbial populations: in situ analyses using rRNA and mRNA stable isotope probing coupled with single-cell raman-fluorescence in situ hybridization.

Abstract:

Prokaryotes represent one-half of the living biomass on Earth, with the vast majority remaining elusive to culture and study within the laboratory. As a result, we lack a basic understanding of the functions that many species perform in the natural world. To address this issue, we developed complementary population and single-cell stable isotope ((13)C)-linked analyses to determine microbial identity and function in situ. We demonstrated that the use of rRNA/mRNA stable isotope probing (SIP) ...

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Publication status:
Published

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Publisher copy:
10.1128/aem.01861-08

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Role:
Author
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Journal:
Applied and environmental microbiology
Volume:
75
Issue:
1
Pages:
234-241
Publication date:
2009-01-01
DOI:
EISSN:
1098-5336
ISSN:
0099-2240
Source identifiers:
62577

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