- Abstract:
-
Prokaryotes represent one-half of the living biomass on Earth, with the vast majority remaining elusive to culture and study within the laboratory. As a result, we lack a basic understanding of the functions that many species perform in the natural world. To address this issue, we developed complementary population and single-cell stable isotope ((13)C)-linked analyses to determine microbial identity and function in situ. We demonstrated that the use of rRNA/mRNA stable isotope probing (SIP) ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1128/aem.01861-08
-
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- Journal:
- Applied and environmental microbiology
- Volume:
- 75
- Issue:
- 1
- Pages:
- 234-241
- Publication date:
- 2009-01-05
- DOI:
- EISSN:
-
1098-5336
- ISSN:
-
0099-2240
- URN:
-
uuid:bf6f7f53-6dea-4f19-a887-32b97e113d38
- Source identifiers:
-
62577
- Local pid:
- pubs:62577
- Copyright date:
- 2009
Journal article
Resolving genetic functions within microbial populations: in situ analyses using rRNA and mRNA stable isotope probing coupled with single-cell raman-fluorescence in situ hybridization.
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