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Critical residues for structure and catalysis in short-chain dehydrogenases/reductases.

Abstract:

Short-chain dehydrogenases/reductases form a large, evolutionarily old family of NAD(P)(H)-dependent enzymes with over 60 genes found in the human genome. Despite low levels of sequence identity (often 10-30%), the three-dimensional structures display a highly similar alpha/beta folding pattern. We have analyzed the role of several conserved residues regarding folding, stability, steady-state kinetics, and coenzyme binding using bacterial 3beta/17beta-hydroxysteroid dehydrogenase and selected...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m202160200

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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Genomics Consortium
Role:
Author
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Journal:
The Journal of biological chemistry
Volume:
277
Issue:
28
Pages:
25677-25684
Publication date:
2002-07-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:bf01b20c-74f8-4f17-87e5-f3381c9db8db
Source identifiers:
108576
Local pid:
pubs:108576

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