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A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs.

Abstract:
An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template interactions during elongation. A C453R mutation in PA can compensate for this defect, suggesting that amino acids C453 and R638 form part of the same domain.
Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
Journal of virology
Volume:
77
Issue:
8
Pages:
5017-5020
Publication date:
2003-04-05
DOI:
EISSN:
1098-5514
ISSN:
0022-538X
URN:
uuid:befb70b7-6120-4401-9f88-c90c117a01c7
Source identifiers:
24505
Local pid:
pubs:24505

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