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Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.

Abstract:

Escherichia coli (E. coli) remains the most commonly used host for recombinant protein expression. It is well known that a variety of experimental factors influence the protein production level as well as the solubility profile of over-expressed proteins. This becomes increasingly important for optimizing production of protein complexes using co-expression strategies. In this study, we focus on the effect of the choice of the expression vector system: by standardizing experimental factors inc...

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Publication status:
Published

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Publisher copy:
10.1016/j.jsb.2011.03.004

Authors


Heidebrecht, T More by this author
Jacobovitch, Y More by this author
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Journal:
Journal of structural biology
Volume:
175
Issue:
2
Pages:
159-170
Publication date:
2011-08-05
DOI:
EISSN:
1095-8657
ISSN:
1047-8477
URN:
uuid:bef0e42e-4719-4c68-a516-a48172d3c00e
Source identifiers:
135171
Local pid:
pubs:135171

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