- Abstract:
-
Escherichia coli (E. coli) remains the most commonly used host for recombinant protein expression. It is well known that a variety of experimental factors influence the protein production level as well as the solubility profile of over-expressed proteins. This becomes increasingly important for optimizing production of protein complexes using co-expression strategies. In this study, we focus on the effect of the choice of the expression vector system: by standardizing experimental factors inc...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.jsb.2011.03.004
-
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- Journal:
- Journal of structural biology
- Volume:
- 175
- Issue:
- 2
- Pages:
- 159-170
- Publication date:
- 2011-08-05
- DOI:
- EISSN:
-
1095-8657
- ISSN:
-
1047-8477
- URN:
-
uuid:bef0e42e-4719-4c68-a516-a48172d3c00e
- Source identifiers:
-
135171
- Local pid:
- pubs:135171
- Copyright date:
- 2011
Journal article
Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.
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