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Journal article

Efficient production and purification of recombinant murine kindlin-3 from insect cells for biophysical studies.

Abstract:

Kindlins are essential coactivators, with talin, of the cell surface receptors integrins and also participate in integrin outside-in signalling, and the control of gene transcription in the cell nucleus. The kindlins are ~75 kDa multidomain proteins and bind to an NPxY motif and upstream T/S cluster of the integrin β-subunit cytoplasmic tail. The hematopoietically-important kindlin isoform, kindlin-3, is critical for platelet aggregation during thrombus formation, leukocyte rolling in respons...

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Journal:
Journal of visualized experiments : JoVE
Issue:
85
Publication date:
2014-01-01
EISSN:
1940-087X
URN:
uuid:be80407a-3884-40f4-9e1f-9487bb268a2e
Source identifiers:
486604
Local pid:
pubs:486604
Language:
English

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