Stochastic detection of Pim protein kinases reveals electrostatically enhanced association of a peptide substrate.

Journal article

In stochastic sensing, the association and dissociation of analyte molecules is observed as the modulation of an ionic current flowing through a single engineered protein pore, enabling the label-free determination of rate and equilibrium constants with respect to a specific binding site. We engineered sensors based on the staphylococcal α-hemolysin pore to allow the single-molecule detection and characterization of protein kinase-peptide interactions. We enhanced this approach by using site-...

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Authors: Harrington, L; Cheley, S; Alexander, LT; Knapp, S; Bayley, H

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 110
• Issue: 47
• Pages: E4417-E4426
• Publication date: 2013-11-01
• DOI: 10.1073/pnas.1312739110
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Kinetics; Protein Binding; Bacterial Toxins; Hemolysin Proteins; Proto-Oncogene Proteins c-pim-1; Stochastic Processes; Static Electricity; Peptides; Signal Transduction; Proteolysis; Protein Engineering