Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFα antagonists.

Journal article

A cell-based assay has been developed for the quantification of the activity of TNFα antagonists based on human erythroleukemic K562 cells transfected with a NFκB regulated firefly luciferase reporter-gene construct. Both drug activity and anti-drug neutralizing antibodies can be quantified with a high degree of precision within 2h, and without interference from cytokines and other factors known to activate NFκB. The assay cells also contain the Renilla luciferase reporter gene under the cont...

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Authors: Lallemand, C; Kavrochorianou, N; Steenholdt, C; Bendtzen, K; Ainsworth, M

• Publication status: Published
• Journal: Journal of immunological methods
• Volume: 373
• Issue: 1-2
• Pages: 229-239
• Publication date: 2011-10-01
• DOI: 10.1016/j.jim.2011.08.022
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Dose-Response Relationship, Drug; Animals; Recombinant Fusion Proteins; NF-kappa B; Antibodies, Monoclonal, Humanized; Gene Expression Regulation, Enzymologic; K562 Cells; Culture Media; Luciferases, Renilla; Luciferases, Firefly; Antibodies, Monoclonal; Crohn Disease; Humans; Serum; Arthritis, Rheumatoid; Tumor Necrosis Factor-alpha; Regulatory Sequences, Nucleic Acid; Antibodies, Neutralizing; Luciferases