Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin.
A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin detection system. Endogenous biotin can interfere with staining systems that employ the use of biotin-avidin- or biotin-streptavidin-based detection systems and may therefore result in high, non-specific background staining. Here, we show that this endog...Expand abstract
- Methods in molecular biology (Clifton, N.J.)
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