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A GFP-based assay reveals a role for RHD3 in transport between the endoplasmic reticulum and Golgi apparatus.

Abstract:

We describe the use of a secreted form of Aequoria victoria green fluorescent protein (secGFP) in a non-invasive live cell assay of membrane traffic in Arabidopsis thaliana. We show that in comparison to GFP-HDEL, which accumulates in the endoplasmic reticulum (ER), secGFP generates a weak fluorescence signal when transported to the apoplast. The fluorescence of secGFP in the apoplast can be increased by growth of seedlings on culture medium buffered at pH 8.1, suggesting that apoplastic pH i...

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Publication status:
Published

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Journal:
The Plant journal : for cell and molecular biology
Volume:
37
Issue:
3
Pages:
398-414
Publication date:
2004-02-05
DOI:
EISSN:
1365-313X
ISSN:
0960-7412
URN:
uuid:bdb4d52b-db8a-4af0-a5bb-9d787c946bc9
Source identifiers:
48863
Local pid:
pubs:48863

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