Leu-574 of human HIF-1alpha is a molecular determinant of prolyl hydroxylation.

Journal article

Hypoxia-inducible factor (HIF)-1alpha, a master regulator of oxygen homeostasis, regulates genes crucial for cell growth and survival. In normoxia, HIF-1alpha is constantly degraded via the ubiquitin-proteasome pathway. The von Hippel-Lindau (VHL) E3 ubiquitin ligase binds HIF-1alpha through specific recognition of hydroxylated Pro-402 or Pro-564, both of which are modified by the oxygen-dependent HIF prolyl hydroxylases (PHDs/HPHs). Despite the identification of a conserved Leu-X-X-Leu-Ala-P...

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Authors: Kageyama, Y; Koshiji, M; To, K; Tian, Y; Ratcliffe, P

• Publication status: Published
• Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• Volume: 18
• Issue: 9
• Pages: 1028-1030
• Publication date: 2004-06-01
• DOI: 10.1096/fj.03-1233fje
• EISSN: 1530-6860
• ISSN: 0892-6638
• Language: English
• Keywords: Leucine; Cell Line; Transcription Factors; Transcription, Genetic; Humans; Cell Line, Tumor; Mutation; Amino Acid Sequence; Hypoxia-Inducible Factor 1, alpha Subunit; Antibodies; Thermodynamics; Procollagen-Proline Dioxygenase; Protein Processing, Post-Translational; Proline; Molecular Sequence Data; Protein Binding; Hydroxylation