Radiation response of cancer stem-like cells from established human cell lines after sorting for surface markers.

Journal article

PURPOSE: A subpopulation of cancer stem-like cells (CSLC) is hypothesized to exist in different cancer cell lines and to mediate radioresistance in solid tumors. METHODS AND MATERIALS: Cells were stained for CSLC markers and sorted (fluorescence-activated cell sorter/magnetic beads) to compare foci and radiosensitivity of phosphorylated histone H2AX at Ser 139 (gamma-H2AX) in sorted vs. unsorted populations in eight cell lines from different organs. CSLC properties were examined using anchora...

Expand abstract

Authors: Al-Assar, O; Muschel, R; Mantoni, T; Mckenna, W; Brunner, T

• Publication status: Published
• Journal: International journal of radiation oncology, biology, physics
• Volume: 75
• Issue: 4
• Pages: 1216-1225
• Publication date: 2009-11-01
• DOI: 10.1016/j.ijrobp.2009.07.001
• EISSN: 1879-355X
• ISSN: 0360-3016
• Language: English
• Keywords: Cell Survival; Cell Line, Tumor; Histones; Cell Separation; Biological Markers; Flow Cytometry; Radiation Tolerance; Tumor Stem Cell Assay; Humans; Female; Neoplastic Stem Cells; Mice, SCID; Mice; Receptor, Notch1; DNA Breaks; Animals; Spheroids, Cellular; Cell Count; Membrane Proteins