Journal article
FAS2FURIOUS: Moderate-throughput secreted expression of difficult recombinant proteins in Drosophila S2 cells
- Abstract:
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Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and the existing low-throughput methodology, use of the Drosophila melanogaster “S2” cell line lags behind more common insect-cell lines like Sf9 or High-Five™. Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represen...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 2.5MB, Terms of use)
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- Publisher copy:
- 10.3389/fbioe.2022.871933
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Bibliographic Details
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Bioengineering and Biotechnology More from this journal
- Volume:
- 10
- Article number:
- 871933
- Publication date:
- 2022-05-05
- Acceptance date:
- 2022-04-04
- DOI:
- EISSN:
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2296-4185
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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1249554
- Local pid:
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pubs:1249554
- Deposit date:
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2022-04-06
Terms of use
- Copyright holder:
- Coker et al.
- Copyright date:
- 2022
- Rights statement:
- ©2022 Coker, Katis, Fairhead, Schwenzer, Clemmensen, Frandsen, De Jongh, Gileadi, Burgess-Brown, Marsden, Midwood and Yue. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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