Journal article icon

Journal article

Overexpression limits of fission yeast cell-cycle regulators in vivo and in silico

Abstract:

Cellular systems are generally robust against fluctuations of intracellular parameters such as gene expression level. However, little is known about expression limits of genes required to halt cellular systems. In this study, using the fission yeast Schizosaccharomyces pombe, we developed a genetic 'tug-of-warg' (gTOW) method to assess the overexpression limit of certain genes. Using gTOW, we determined copy number limits for 31 cell-cycle regulators; the limits varied from 1 to >100. Comp...

Expand abstract

Actions


Access Document


Publisher copy:
10.1038/msb.2011.91

Authors


Journal:
Molecular Systems Biology
Volume:
7
Publication date:
2011-01-01
DOI:
EISSN:
1744-4292
URN:
uuid:bce9b104-6b62-4888-860b-c11a3e47f53d
Source identifiers:
222415
Local pid:
pubs:222415
Language:
English

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP