Enhanced immunogenicity and protective efficacy against Mycobacterium tuberculosis of bacille Calmette-Guerin vaccine using mucosal administration and boosting with a recombinant modified vaccinia virus Ankara.

Journal article

Heterologous prime-boost immunization strategies can evoke powerful T cell immune responses and may be of value in developing an improved tuberculosis vaccine. We show that recombinant modified vaccinia virus Ankara, expressing Mycobacterium tuberculosis Ag 85A (M.85A), strongly boosts bacille Calmette-Guérin (BCG)-induced Ag 85A specific CD4(+) and CD8(+) T cell responses in mice. A comparison of intranasal (i.n.) and parenteral immunization of BCG showed that while both routes elicited comp...

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Authors: Goonetilleke, N; Mcshane, H; Hannan, C; Anderson, R; Brookes, R

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 171
• Issue: 3
• Pages: 1602-1609
• Publication date: 2003-08-01
• DOI: 10.4049/jimmunol.171.3.1602
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Vaccinia virus; Immunodominant Epitopes; Acyltransferases; Administration, Intranasal; CD4-Positive T-Lymphocytes; Immunization Schedule; Mice; Antigens, Bacterial; Nasal Mucosa; Viral Vaccines; Animals; Female; Lymph Nodes; Spleen; BCG Vaccine; CD8-Positive T-Lymphocytes; Mice, Inbred BALB C; Injections, Intradermal; Lung; Immunization, Secondary; Vaccines, Synthetic; Adjuvants, Immunologic; Molecular Sequence Data; Amino Acid Sequence; Cells, Cultured; T-Lymphocyte Subsets; Dose-Response Relationship, Immunologic; Mycobacterium tuberculosis