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Detection of monoclonal antibodies against cell surface antigens: the use of antiglobulins coupled to red blood cells.

Abstract:

An antiglobulin-coupled red cell assay is described for screening monoclonal antibodies against cell surface antigens. A monoclonal antibody specific for rat immunoglobulin kappa chains was coupled to red blood cells and used to detect binding of rat monoclonal antibodies to cells attached to the wells of microtitre plates. The method was found to be simpler and more rapid than the alternative enzyme-linked binding assay and useful for rapid screening and selection of antibodies for use as di...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Coombs, RR More by this author
Journal:
Journal of immunological methods
Volume:
66
Issue:
1
Pages:
81-87
Publication date:
1984-01-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:bc27f192-a5aa-4d2e-acb3-aac3cd96b432
Source identifiers:
17186
Local pid:
pubs:17186

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