Journal article
Purification and characterization of MxB
- Abstract:
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MxB/Mx2 is an interferon-induced dynamin-like GTPase, which restricts a number of life-threatening viruses. Because of its N-terminal region, predicted to be intrinsically disordered, and its propensity to self-oligomerize, purification of the full-length protein has not been successful in conventional E. coli expression systems. In this chapter, we describe an expression and purification procedure to obtain pure full-length wild-type MxB from suspension-adapted mammalian cells. We further de...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, 286.2KB, Terms of use)
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- Publisher copy:
- 10.1007/978-1-0716-0676-6_5
Authors
Funding
Bibliographic Details
- Publisher:
- Springer Nature
- Series:
- Methods in Molecular Biology
- Journal:
- Dynamin Superfamily GTPases More from this journal
- Volume:
- 2159
- Pages:
- 55-65
- Publication date:
- 2020-06-12
- DOI:
- EISSN:
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1940-6029
- ISSN:
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1064-3745
- Pmid:
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32529363
Item Description
- Language:
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English
- Keywords:
- Pubs id:
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1113597
- Local pid:
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pubs:1113597
- Deposit date:
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2021-07-14
Terms of use
- Copyright holder:
- Springer
- Copyright date:
- 2020
- Rights statement:
- © Springer Science+Business Media, LLC, part of Springer Nature 2020, corrected publication 2020 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed.
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from Springer Nature at: https://doi.org/10.1007/978-1-0716-0676-6_5
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