An efficient method for purification of human T-cell growth factor.

Journal article

A 1000-fold purification of human T-cell growth factor (TCGF) was achieved starting from supernatants of human spleen cells stimulated with phytohaemagglutinin (PHA) in culture medium containing 0.5% serum. The purification scheme involved precipitation with ammonium sulphate, gel filtration and blue-Sepharose chromatography. The use of polyethylene glycol 6000 (PEG 6000) was critical during the chromatographic steps in order to obtain high final recoveries or activity (40-50%). Purified preparations of TCGF labelled with 125I by the chloramine T method revealed that the activity co-migrated with 2 molecular species of 14,000-17,000 daltons in SDS-PAGE under non-reducing conditions.

Authors: Acuto, O; Cianfriglia, M; Colombatti, M; Chapuis, B; Nabholz, M

• Publication status: Published
• Journal: Journal of immunological methods
• Volume: 53
• Issue: 1
• Pages: 15-26
• Publication date: 1982-08-01
• DOI: 10.1016/0022-1759(82)90236-8
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Chromatography, Agarose; T-Lymphocytes, Cytotoxic; Interleukin-2; Humans; Cell Line; Mice; Culture Media; Spleen; Precipitation; Electrophoresis, Polyacrylamide Gel; Chromatography, Gel; Animals; Dialysis