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A facile method for reversibly linking a recombinant protein to DNA.

Abstract:
We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.
Publication status:
Published

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Publisher copy:
10.1002/cbic.200900165

Authors


Goodman, RP More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
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Journal:
Chembiochem : a European journal of chemical biology
Volume:
10
Issue:
9
Pages:
1551-1557
Publication date:
2009-06-05
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
URN:
uuid:bb15c938-7139-4196-81e3-70811478e1b4
Source identifiers:
29541
Local pid:
pubs:29541

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