Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.

Journal article

One of the major DNA interstrand cross-link (ICL) repair pathways in mammalian cells is coupled to replication, but the mechanistic roles of the critical factors involved remain largely elusive. Here, we show that purified human SNM1A (hSNM1A), which exhibits a 5'-3' exonuclease activity, can load from a single DNA nick and digest past an ICL on its substrate strand. hSNM1A-depleted cells are ICL-sensitive and accumulate replication-associated DNA double-strand breaks (DSBs), akin to ERCC1-de...

Expand abstract

Authors: Wang, A; Sengerová, B; Cattell, E; Inagawa, T; Hartley, J

• Publication status: Published
• Journal: Genes and development
• Volume: 25
• Issue: 17
• Pages: 1859-1870
• Publication date: 2011-09-01
• DOI: 10.1101/gad.15699211
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: Nuclear Proteins; DNA; Hela Cells; Endonucleases; DNA Breaks, Double-Stranded; DNA Repair; Cell Line, Tumor; DNA Repair Enzymes; DNA-Binding Proteins; Humans; DNA Breaks, Single-Stranded