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Molecular typing of Leptospira spp. based on putative O-antigen polymerase gene (wzy), the benefit over 16S rRNA gene sequence.

Abstract:

Molecular typing of leptospiral strains based on variation within putative O-antigen polymerase gene (wzy) was determined among reference strains and those isolated from patients. Using the PCR primers designed from the flanking gene of wzy derived from Leptospira interrogans serovar Copenhageni, all L. interrogans serovars as well as human and rodent leptospiral isolates from Thailand could be amplified. The size of PCR product ranged from 1 to 1.5 kb. The limitation of these primer pairs wa...

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Publication status:
Published

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Journal:
FEMS microbiology letters
Volume:
271
Issue:
2
Pages:
170-179
Publication date:
2007-06-05
DOI:
EISSN:
1574-6968
ISSN:
0378-1097
URN:
uuid:ba9e5be6-bc19-4178-9221-9acd247c251f
Source identifiers:
1078
Local pid:
pubs:1078

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