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EV-mediated promotion of myogenic differentiation is dependent on dose, collection medium, and isolation method

Abstract:

Extracellular vesicles (EVs) have been implicated in the regulation of myogenic differentiation. C2C12 murine myoblast differentiation was reduced following treatment with GW4869 or heparin (to inhibit exosome biogenesis and EV uptake, respectively). Conversely, treatment with C2C12 myotube-conditioned medium enhanced myogenic differentiation. Ultrafiltration-size exclusion liquid chromatography (UF-SEC) was used to isolate EVs and non-EV extracellular protein in parallel from C2C12 myobla...

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Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1016/j.omtn.2023.07.005

Authors


Publisher:
Cell Press
Journal:
Molecular Therapy - Nucleic Acids More from this journal
Volume:
33
Pages:
511-528
Publication date:
2023-07-14
Acceptance date:
2023-07-11
DOI:
EISSN:
2162-2531
Pmid:
37602275
Language:
English
Keywords:
Pubs id:
1510253
Local pid:
pubs:1510253
Deposit date:
2023-09-21

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