We describe the use of nano-electrospray ionization (nano-ESI) mass spectrometry (MS) for monitoring hydrogen exchange. Using this approach, we have compared the fluctuations in structure of the wild-type human lysozyme with those of the Asp67His and Ile56Thr variants, the two amyloidogenic forms of the protein. The results revealed that a significant region of the structure was transiently unfolded in both variants compared with the wild-type protein. Using peptic digestion, we located the r...Expand abstract
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Site-specific hydrogen exchange of proteins: insights into the structures of amyloidogenic intermediates.
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