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Journal article

Site-specific hydrogen exchange of proteins: insights into the structures of amyloidogenic intermediates.

Abstract:

We describe the use of nano-electrospray ionization (nano-ESI) mass spectrometry (MS) for monitoring hydrogen exchange. Using this approach, we have compared the fluctuations in structure of the wild-type human lysozyme with those of the Asp67His and Ile56Thr variants, the two amyloidogenic forms of the protein. The results revealed that a significant region of the structure was transiently unfolded in both variants compared with the wild-type protein. Using peptic digestion, we located the r...

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Publication status:
Published

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Journal:
Methods in enzymology
Volume:
402
Pages:
389-402
Publication date:
2005-01-01
DOI:
EISSN:
1557-7988
ISSN:
0076-6879
URN:
uuid:b8ea61e6-d958-4de4-bc26-41a32d8a0e02
Source identifiers:
59282
Local pid:
pubs:59282

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