Journal article
Site-specific hydrogen exchange of proteins: insights into the structures of amyloidogenic intermediates.
- Abstract:
-
We describe the use of nano-electrospray ionization (nano-ESI) mass spectrometry (MS) for monitoring hydrogen exchange. Using this approach, we have compared the fluctuations in structure of the wild-type human lysozyme with those of the Asp67His and Ile56Thr variants, the two amyloidogenic forms of the protein. The results revealed that a significant region of the structure was transiently unfolded in both variants compared with the wild-type protein. Using peptic digestion, we located the r...
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- Publication status:
- Published
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- Publisher copy:
- 10.1016/s0076-6879(05)02012-4
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Authors
Bibliographic Details
- Journal:
- Methods in enzymology
- Volume:
- 402
- Pages:
- 389-402
- Publication date:
- 2005-01-01
- DOI:
- EISSN:
-
1557-7988
- ISSN:
-
0076-6879
- Source identifiers:
-
59282
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:59282
- UUID:
-
uuid:b8ea61e6-d958-4de4-bc26-41a32d8a0e02
- Local pid:
- pubs:59282
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2005
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