- Abstract:
-
We describe the use of nano-electrospray ionization (nano-ESI) mass spectrometry (MS) for monitoring hydrogen exchange. Using this approach, we have compared the fluctuations in structure of the wild-type human lysozyme with those of the Asp67His and Ile56Thr variants, the two amyloidogenic forms of the protein. The results revealed that a significant region of the structure was transiently unfolded in both variants compared with the wild-type protein. Using peptic digestion, we located the r...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/s0076-6879(05)02012-4
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- Journal:
- Methods in enzymology
- Volume:
- 402
- Pages:
- 389-402
- Publication date:
- 2005-01-01
- DOI:
- EISSN:
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1557-7988
- ISSN:
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0076-6879
- URN:
-
uuid:b8ea61e6-d958-4de4-bc26-41a32d8a0e02
- Source identifiers:
-
59282
- Local pid:
- pubs:59282
- Copyright date:
- 2005
Journal article
Site-specific hydrogen exchange of proteins: insights into the structures of amyloidogenic intermediates.
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