Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension.

Journal article

Current methods used to genotype point mutations in Plasmodium falciparum genes involved in resistance to antifolate drugs include restriction digestion of PCR products, allele-specific amplification or sequencing. Here we demonstrate that known point mutations in dihydrofolate reductase and dihydropteroate synthase can be scored quickly and accurately by single-nucleotide primer extension and detection of florescent products on a capillary sequencer. We use this method to genotype parasites ...

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Authors: Nair, S; Brockman, A; Paiphun, L; Nosten, F; Anderson, T

• Publication status: Published
• Journal: International journal for parasitology
• Volume: 32
• Issue: 7
• Pages: 852-858
• Publication date: 2002-06-01
• DOI: 10.1016/s0020-7519(02)00033-4
• EISSN: 1879-0135
• ISSN: 0020-7519
• Language: English
• Keywords: Plasmodium falciparum; Animals; Malaria, Falciparum; Polymorphism, Single Nucleotide; Humans; Tetrahydrofolate Dehydrogenase; DNA, Protozoan; Drug Resistance; Dihydropteroate Synthase; Polymerase Chain Reaction; Thailand; Folic Acid Antagonists; Sequence Analysis, DNA; Point Mutation; Myanmar; Antimalarials