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Sensitive and specific enzymatic assay for the determination of precursor forms of prostate-specific antigen after an activation step.

Abstract:

BACKGROUND: The proteome of the serine protease prostate-specific antigen (PSA) and its enzymatic properties have been clarified only recently. We have developed a specific and sensitive method for the measurement of active PSA and used it to measure proPSA in blood. METHODS: We used the synthetic peptide KGISSQY, which possesses a PSA-specific cleavage site, as substrate. To ascertain the specificity of the assay, we used an anti-PSA monoclonal antibody that captures known forms of PSA. An ...

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Institution:
University of Oxford
Department:
Oxford, MSD, Surgical Sciences
Role:
Author
Journal:
Clinical chemistry
Volume:
48
Issue:
8
Pages:
1257-1264
Publication date:
2002-08-05
EISSN:
1530-8561
ISSN:
0009-9147
URN:
uuid:b841d4fd-43e5-4512-b5c2-e50ddfaaddd9
Source identifiers:
366025
Local pid:
pubs:366025

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