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Journal article

Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Abstract:

Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41587-021-00965-w

Authors


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Role:
Author
ORCID:
0000-0002-8607-1226
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
ORCID:
0000-0002-6538-3036
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Name:
Medical Research Council
Funder identifier:
http://dx.doi.org/10.13039/501100000265
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Name:
Medical Research Foundation
Publisher:
Springer Nature
Journal:
Nature Biotechnology More from this journal
Volume:
39
Pages:
1517-1520
Publication date:
2021-07-01
Acceptance date:
2021-05-21
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
Pmid:
34211161
Language:
English
Keywords:
Pubs id:
1184826
Local pid:
pubs:1184826
Deposit date:
2021-07-04

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