Journal article icon

Journal article

Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore.

Abstract:

The staphylococcal alpha-hemolysin (alphaHL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although discrimination of all four bases of DNA by the alphaHL pore has been demonstrated, analysis of single-stranded DNAs and RNAs containing secondary structure mediated by basepairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1016/j.bpj.2009.12.4333

Authors


Journal:
Biophysical journal
Volume:
98
Issue:
9
Pages:
1856-1863
Publication date:
2010-05-05
DOI:
EISSN:
1542-0086
ISSN:
0006-3495
URN:
uuid:b7711764-af33-4348-a68f-9b9df6426abe
Source identifiers:
54681
Local pid:
pubs:54681

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP