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Strategies for the compensation of specimen-induced spherical aberration in confocal microscopy of skin.

Abstract:

We consider various strategies for confocal imaging of human skin which seek to reduce the effects of the specimen-induced aberrations. We calculate the spherical aberration introduced by the stratified structure of skin and show how the confocal signal is affected when attempting to image at various depths within the dermis. Using simple methods it is shown how images might be improved by compensating for the induced aberration. The methods include the use of an iris to reduce the pupil area...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Journal:
Journal of microscopy
Volume:
200
Issue:
Pt 1
Pages:
68-74
Publication date:
2000-10-05
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
URN:
uuid:b6f29e22-6181-4669-a3db-3158fb01248c
Source identifiers:
62390
Local pid:
pubs:62390

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