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The superhelical density of nuclear DNA from human cells.

Abstract:

Structures resembling nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These structures, which we call nucleoids, sediment in sucrose gradients containing the intercalating agent, actinomycin D, in the manner characteristic of superhelical DNA. We have determined the concentration of actinomycin that minimises the rate of sedimentation of nucleoids. At this concentration, we have determined the amount of drug bou...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
European journal of biochemistry / FEBS
Volume:
74
Issue:
3
Pages:
527-531
Publication date:
1977-04-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:b6e5f1fb-a1e8-4efe-a0a3-31a2eb20b4e7
Source identifiers:
25782
Local pid:
pubs:25782

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