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Triplet-relaxation microscopy with bunched pulsed excitation.

Abstract:

Obtaining high signal levels in fluorescence microscopy is usually spoiled by the concomitant population of the dark (triplet) state of the marker, which is often followed by photobleaching. Recently, we introduced the triplet relaxation (T-Rex) modality in fluorescence microscopy which led to a major increase in total signal and dye photostability. The idea behind T-Rex is to avoid the illumination of fluorophores in the triplet state, e.g. by using pulsed excitation with interpulse time dis...

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Publication status:
Published

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Publisher copy:
10.1039/b903357m

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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Journal:
Photochemical and photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Volume:
8
Issue:
4
Pages:
481-485
Publication date:
2009-04-01
DOI:
EISSN:
1474-9092
ISSN:
1474-905X
Source identifiers:
222191
Language:
English
Keywords:
Pubs id:
pubs:222191
UUID:
uuid:b6c3db20-15ce-440f-aabc-6c25a8d59ed2
Local pid:
pubs:222191
Deposit date:
2012-12-19

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