Journal article
Rapid formation of non-native contacts during the folding of HPr revealed by real-time photo-CIDNP NMR and stopped-flow fluorescence experiments.
- Abstract:
-
We report the combined use of real-time photo-CIDNP NMR and stopped-flow fluorescence techniques to study the kinetic refolding of a set of mutants of a small globular protein, HPr, in which each of the four phenylalanine residues has in turn been replaced by a tryptophan residue. The results indicate that after refolding is initiated, the protein collapses around at least three, and possibly all four, of the side-chains of these residues, as (i) the observation of transient histidine photo-C...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of molecular biology
- Volume:
- 330
- Issue:
- 2
- Pages:
- 397-407
- Publication date:
- 2003-07-01
- DOI:
- EISSN:
-
1089-8638
- ISSN:
-
0022-2836
- Source identifiers:
-
32394
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:32394
- UUID:
-
uuid:b67acf9d-dce8-43fa-a473-8f26476875ed
- Local pid:
- pubs:32394
- Deposit date:
- 2012-12-19
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- Copyright date:
- 2003
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