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Precise probing of residue roles by post-translational β,γ-C,N aza-Michael mutagenesis in enzyme active sites.

Abstract:

Biomimicry valuably allows the understanding of the essential chemical components required to recapitulate biological function, yet direct strategies for evaluating the roles of amino acids in proteins can be limited by access to suitable, subtly-altered unnatural variants. Here we describe a strategy for dissecting the role of histidine residues in enzyme active sites using unprecedented, chemical, post-translational side-chain-β,γ C-N bond formation. Installation of dehydroalanine (as a "ta...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Version of Record

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Publisher copy:
10.1021/acscentsci.7b00341

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Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
More by this author
Institution:
University of Oxford
Division:
MPLS Division
Department:
Chemistry; Organic Chemistry
ORCID:
0000-0001-6594-8917
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Grant:
CTQ2014-55174-P
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Publisher:
ACS Publications Publisher's website
Journal:
ACS Central Science Journal website
Volume:
3
Issue:
11
Pages:
1168-1173
Publication date:
2017-11-13
DOI:
EISSN:
2374-7951
ISSN:
2374-7943
Pubs id:
pubs:809687
URN:
uri:b654e241-1255-4019-8004-205a2c195072
UUID:
uuid:b654e241-1255-4019-8004-205a2c195072
Local pid:
pubs:809687
Language:
English
Keywords:

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