Precise probing of residue roles by post-translational β,γ-C,N aza-Michael mutagenesis in enzyme active sites.
Biomimicry valuably allows the understanding of the essential chemical components required to recapitulate biological function, yet direct strategies for evaluating the roles of amino acids in proteins can be limited by access to suitable, subtly-altered unnatural variants. Here we describe a strategy for dissecting the role of histidine residues in enzyme active sites using unprecedented, chemical, post-translational side-chain-β,γ C-N bond formation. Installation of dehydroalanine (as a "ta...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
- Version of Record
- ACS Publications Publisher's website
- ACS Central Science Journal website
- Publication date:
- Pubs id:
- Local pid:
- Copyright holder:
- American Chemical Society
- Copyright date:
- Copyright © 2017 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.