1,25-Dihydroxyvitamin D(3) and prostaglandin E(2) act directly on circulating human osteoclast precursors.

Journal article

1,25-Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) and prostaglandin E(2) (PGE(2)) are known to influence osteoclast formation indirectly through their effects on osteoblasts. To determine whether 1, 25(OH)(2)D(3) and PGE(2) also have a direct effect on circulating osteoclast precursors, these factors were added to long-term cultures of human peripheral blood mononuclear cells (PBMCs) in the presence of osteoprotegerin ligand and macrophage colony-stimulating factor (M-CSF) (+/-dexamethasone). The ...

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Authors: Itonaga, I; Sabokbar, A; Neale, S; Athanasou, N

• Publication status: Published
• Journal: Biochemical and biophysical research communications
• Volume: 264
• Issue: 2
• Pages: 590-595
• Publication date: 1999-10-01
• DOI: 10.1006/bbrc.1999.1545
• EISSN: 1090-2104
• ISSN: 0006-291X
• Language: English
• Keywords: Osteoclasts; Osteoprotegerin; Cholecalciferol; Glycoproteins; Macrophage Colony-Stimulating Factor; Cells, Cultured; Cell Differentiation; Dexamethasone; Receptors, Tumor Necrosis Factor; Receptors, Cytoplasmic and Nuclear; Monocytes; Dinoprostone; Humans