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ORF6 from the clavulanic acid gene cluster of Streptomyces clavuligerus has ornithine acetyltransferase activity.

Abstract:

The clinically used beta-lactamase inhibitor clavulanic acid is produced by fermentation of Streptomyces clavuligerus. The orf6 gene of the clavulanic acid biosynthetic gene cluster in S. clavuligerus encodes a protein that shows sequence homology to ornithine acetyltransferase (OAT), the fifth enzyme of the arginine biosynthetic pathway. Orf6 was overexpressed in Escherichia coli (at approximately 15% of total soluble protein by SDS/PAGE analysis) indicating it was not toxic to the host cell...

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Publication status:
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Authors


Kershaw, NJ More by this author
McNaughton, HJ More by this author
Hewitson, KS More by this author
Hernández, H More by this author
Griffin, J More by this author
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Journal:
European journal of biochemistry / FEBS
Volume:
269
Issue:
8
Pages:
2052-2059
Publication date:
2002-04-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:b5a7817b-6465-43fc-b7c1-3a7e68770637
Source identifiers:
32099
Local pid:
pubs:32099

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