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Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3.

Abstract:

OBJECTIVE: To develop a simple and efficient method for producing homogeneous populations of monocytes and macrophages from human embryonic stem cells (hES). MATERIALS AND METHODS: Human embryonic stem cell lines KCL001, KCL002, and HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth factors using a three-step method. The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and int...

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Publication status:
Published

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Journal:
Experimental hematology
Volume:
36
Issue:
9
Pages:
1167-1175
Publication date:
2008-09-01
DOI:
EISSN:
1873-2399
ISSN:
0301-472X
Source identifiers:
24818
Language:
English
Keywords:
Pubs id:
pubs:24818
UUID:
uuid:b5592f18-06a1-449c-827a-f5b8e83f1242
Local pid:
pubs:24818
Deposit date:
2012-12-19

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