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Journal article

Affinity, kinetics, and thermodynamics of E-selectin binding to E-selectin ligand-1.

Abstract:

E-selectin is an endothelial adhesion molecule, which mediates the tethering and rolling of leukocytes on vascular endothelium. It recognizes the glycoprotein E-selectin ligand-1 (ESL-1) as a major binding partner on mouse myeloid cells. Using surface plasmon resonance, we measured the kinetics and affinity of binding of monomeric E-selectin to ESL-1 isolated from mouse bone marrow cells. E-selectin bound to ESL-1 with a fast dissociation rate constant of 4.6 s(-1) and a calculated associatio...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m104844200

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
The Journal of biological chemistry
Volume:
276
Issue:
34
Pages:
31602-31612
Publication date:
2001-08-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:b3c2064b-097a-4cbd-bfa2-c9370a22f9f1
Source identifiers:
22292
Local pid:
pubs:22292

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